Entering an email address is optional. If you provide an email address, a link to the results webpage will be send to that email. Your email address will not be shared with any third parties.
Sequence input can be provided by copying sequence in the text field or by uploading a fasta file. TopProperty predicts only protein properties, therefore every symbol not corresponding to a capital letter amino acid symbol is turned into an alanine ('A'). Fasta headers are removed. The length of the entered sequence must be between 30 and 1000 residues. The server will only accept single sequences, i.e. the first sequence in fasta file. If you want to model multiple proteins, please submit the sequences separately.
An optional job name can be set. The downloadable results files will then have the job name as an identifier. The job name must not be longer than 30 characters and may only consist of letters, numbers, and the underscore ('_') symbol.
If you want to try out the Server without providing a sequence, three example runs are prepared.
This is the amino-acid sequence of the protein colored according to amino-acid properties based on the ClustalW coloring scheme.
This is a sequence of numbers representing the confidence of the secondary structure (SS) classification. The maximum value is 9 which represents more than 95% confidence.
The minimum value is 3 and represents less than 40% confidence. The coloring corresponds to the predicted 3-state secondary structure labels:
- Helical residues are red.
- Strand residues are yellow.
- Coil residues are green.
This is a sequence of numbers representing the confidence of the solvent accessibility (SA) classification. The maximum value is 9 which represents more than 95% confidence.
The minimum value is 3 and represents less than 40% confidence. The coloring corresponds to the predicted 3-state solvent accessibility labels:
- Buried residues (Relative solvent accessibility < 10%) are colored magenta.
- Partially exposed (Relative solvent accessibility 10-40%) are colored blue.
- Fully exposed residues (Relative solvent accessibility > 40%) are colored orange.
This is a sequence of numbers representing the relative solvent accessibility (RSA) prediction scaled from 0 to 9. The coloring corresponds to a gradient of solvent accessibility:
- Residues with a relative solvent accessibility < 50% are colored shades of red.
- Residues with a relative solvent accessibility > 50% are colored shades of blue.
This is a sequence of numbers representing the confidence of the trans-membrane topology (TMT) prediction. The maximum value is 9 which represents more than 95% confidence.
The minimum value is 3 and represents less than 40% confidence. The coloring corresponds to the predicted 3-state topology prediction:
- Residues inside the cell are colored orange.
- Residues in the membrane are colored red.
- Residues outside the cell are colored blue.
This is a sequence of numbers representing the confidence of the membrane exposure (ME) classification. The maximum value is 9 which represents more than 95% confidence.
The minimum value is 5 and represents less than 60% confidence.
- Membrane exposed residues (Membrane exposure > 50%) are colored magenta.
- Not exposed residues (Membrane exposure < 50%) are colored light green.
This is a sequence of numbers representing the relative membrane exposure (RME) prediction scaled from 0 to 9. The coloring corresponds to a gradient of membrane exposure:
- Residues with a relative membrane exposure < 50% are colored shades of red.
- Residues with a relative membrane exposure > 50% are colored shades of blue.
If you have any questions or suggestions, please write an email to cpcweb[at]uni-duesseldorf.de.